![](https://www.ingenza.com/wp-content/themes/ingenza2022/images/hosts/bacillus.sp.jpg)
Host
- B. subtilis – GRAS, endotoxin-free host, HCD fed-batch fermentation
- Efficient secretion of heterologous proteins
![](https://www.ingenza.com/wp-content/themes/ingenza2022/images_2022/case-studies/case-study-5/diagram1.png)
Build
- Multiple signal peptide gene sequence configurations
![](https://www.ingenza.com/wp-content/themes/ingenza2022/images_2022/case-studies/case-study-5/diagram2.jpg)
Screen
- Signal peptide enabling optimal VLP secretion identified
- Optimal host protease knockout combination identified
![](https://www.ingenza.com/wp-content/themes/ingenza2022/images_2022/case-studies/case-study-5/diagram3.jpg)
Pre-clinical testing
- Immunological equivalence of Bacillus derived VLP confirmed
- Progressed to successful pre-clinical animal testing