
Host
- B. subtilis – GRAS, endotoxin-free host, HCD fed-batch fermentation
- Efficient secretion of heterologous proteins

Build
- Multiple signal peptide gene sequence configurations

Screen
- Signal peptide enabling optimal VLP secretion identified
- Optimal host protease knockout combination identified

Pre-clinical testing
- Immunological equivalence of Bacillus derived VLP confirmed
- Progressed to successful pre-clinical animal testing