Case study: Design-of-Experiment guided protein solubility optimisation

Customer problem

  • Increase yield of target enzyme in soluble active form for commercial application
  • Customer unable to improve upon in-house process

Ingenza solution

  • Novel host/process with 3x increased soluble recombinant protein production
  • Scalable biomanufacturing process meeting customer specifications

Results comparison

Customer benchmark

Target mostly insoluble/inactive

Final process

3x increased soluble/active
Host
  • B. subtilis production host
  • HCD fermentation
Build
  • Automated robotic assembly of genetic configurations
  • Iterative revision to optimise target expression
Screen
  • Expression optimised using DoE guidance
  • Parameter modelling to maximise target yield/solubility
Scale
  • Optimisation of culture medium/process
  • Scalable batch culture

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