Case study: Screen for optimized protein expression

Customer problem

  • Therapeutic protein manufactured using recombinant E. coli contaminated with truncated products due to incomplete translation of the target gene

Ingenza solution

  • Design and construct library of ~50,000 variants of the gene target
  • High-throughput solid phase screen and liquid phase assay to confirm optimised full-length protein production, free of truncated contaminants
  • Optimised gene sequences provided to customer
  • Recombinant E. coli used to express target protein therapeutic
  • Design/construct codon-variant library
  • Library fused to gene encoding visible reporter
  • Solid-phase screen identified best expressed clones
  • Liquid phase assay confirmed productivity of target
  • Reporter removed, purity of target characterised

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